endocan在脓毒症致早期急性呼吸窘迫综合征小鼠肺泡灌洗液中的表达-现代医学

endocan在脓毒症致早期急性呼吸窘迫综合征小鼠肺泡灌洗液中的表达

单位：南京医科大学附属儿童医院, 江苏南京 210008

分类号：R-33;R563.8

出版年·卷·期（页码）：2018·46·第五期（510-513）

摘要：

目的：检测脓毒症致早期急性呼吸窘迫综合征（ARDS）小鼠肺泡灌洗液中endocan的表达。方法：将40只SPF级6~8周的C57BL/6小鼠随机分为实验组（CLP组）和对照组（Sham组），分别进行盲肠结扎穿孔术（CLP）和假手术，术后24 h收取小鼠肺泡灌洗液，采用Elisa法检测endocan、肿瘤坏死因子（TNF-α）、白介素-6（IL-6）、白介素-18（IL-18）、干扰素-γ（IFN-γ）的含量，并分析endocan与IFN-γ、IL-6、IL-18、TNF-α之间的相关性。结果：CLP组小鼠肺泡灌洗液中endocan、IL-6、IL-18、TNF-α、IFN-γ的含量明显高于Sham组，差异均有统计学意义（P<0.05）。endocan与IFN-γ、IL-6、IL-18、TNF-α水平呈正相关（均P<0.05）。结论：脓毒症致早期ARDS小鼠肺泡灌洗液中endocan表达显著升高，提示endocan可作为脓毒症致早期ARDS潜在的生物标志物。

Objective: To establish sepsis induced early acute respiratory distress syndrome(ARDS) mouse model by cecal ligation and puncture (CLP) and to observe the expression of endocan in the bronchoalveolar lavage fluid(BALF).Methods: Forty SPF C57BL/6 mouse aged 6-8 weeks were randomly divided into two groups, experimental group (CLP group) and control group (Sham group) for CLP and sham operation respectively. After 24 h, the levels of endocan, IL-6, IL-18, TNF-α and IFN-γ in BALF were measured by Elisa kit. The correlation between endocan and IL-6, IL-18, TNF-α, IFN-γ were analyzed.Results: Compared with Sham group, the levels of endocan, IL-6, IL-18, TNF-α and IFN-γ in BALF in the CLP group were significantly higher(P<0.05).All the difference were statistically significant on the expression.The levels of endocan had significant positive correlation with IL-6, IL-18, TNF-α, IFN-γ respectively.Conclusion: The endocan expression in BALF is increased in mouse with early ARDS induced by sepsis, suggesting that endocan may be a potential biomarker for early ARDS caused by sepsis.

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