Objective: To explore the expression of serum MALAT-1 in prostate cancer patients and its diagnostic value.Methods: A total of eighty-five prostate cancer patients, sixty-eight benign prostatic hyperplasia and fifty male healthy person were selected as cancer group, benign group and control group, respectively, the vein blood in the cancer group and the benign group was collected at pre-operation, 3-day post-operation and 7-day post-operation, respectively. Meanwhile, same volume of vein blood was collected in the control group. The serum levels of MALAT-1 was detected by quantitative real-time PCR, the correlation between MALAT-1 and patients clinical parameters, as well as its diagnostic value were analyzed.Results: The expression of serum MALAT-1 in the cancer group was significantly higher than in the benign group and the control group (P<0.01), however, there was no significant difference between the benign group and the control group (P>0.05). Compared with pre-operation, there were no changes of MALAT-1 expression at 3-day and 7-day post-operation in the benign group (P>0.05), but at 3-day post-operation, the expression of MALAT-1 was obviously increased in the cancer group when compared to the pre-operation (P<0.01). The expression of MALAT-1 in the T2a group was significantly higher than in the T2a group (P<0.001), the expression of MALAT-1 in the group Gleason scores>7 was significantly higher than in the group Gleason scores ≤ 7 (P<0.001), the expression of MALAT-1 in the bone metastases group was significantly higher than that in the without bone metastases group (P<0.001), there was no significant correlation between MALAT-1 and age, PSA (P>0.05). The area under curve of combined diagnostic of MALAT-1 and PSA (0.865) was higher than single MALAT-1 (0.759) or PSA (0.800). The AUC of MALAT-1 for PSA-negtive prostate cancer was 0.747 (95% CI:0.650-0.829), and the specificity and sensitivity were 76.4% and 66.2%, respectively.Conclusion: MALAT-1 has a good diagnostic value and can be used as a new non-invasive biomarker for the diagnosis of prostate cancer. |
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