Objective: To investigate the effect of high lipid diet on the expression of inflammatory factor oncostatin-M(OSM) in rat kidney.Methods: The healthy male Wistar rats were randomly divided into 4 groups:normal diet group, high fat diet group fed for 4 weeks, high fat diet fed for 8 weeks and high fat diet for 12 weeks. The expression of OSM in renal tissue was detected by immunoblotting. The expression of OSM in serum was detected by ELISA. The biochemical method was used to detect renal function, blood lipid and 24-hour urine protein, and the correlation between OSM expression and each index was analyzed. Results: The expression of OSM in hyperlipidemia rats was significantly higher than that in control group(P<0.05), and with the prolongation of high-fat diet, the expression of OSM also increased significantly(P<0.05). There was a significant and positive correlation between OSM and renal function, blood lipid and 24-hour urine protein(P<0.05). Conclusion: The expression of OSM in high lipid rats increases significantly, which is correlated with renal function, blood lipid and 24-hour urine protein, suggesting that OSM expression is likely to be involved in the pathogenesis of renal injury induced by high lipid. |
