TURBT联合吡柔比星灌注对浅表性膀胱癌肿瘤细胞活力及mTOR通路的影响-现代医学

TURBT联合吡柔比星灌注对浅表性膀胱癌肿瘤细胞活力及mTOR通路的影响

单位：邛崃市医疗中心医院泌尿外科, 四川成都 611530

分类号：R737.14

出版年·卷·期（页码）：2017·36·第七期（933-937）

摘要：

目的：分析经尿道膀胱肿瘤切除术（TURBT）联合吡柔比星灌注治疗浅表性膀胱癌患者后，患者肿瘤细胞活力和信号通路因子表达的影响。方法：将2010年1月至2015年1月间我院收治的113例浅表性膀胱癌患者分为观察组和对照组两组，观察组采用TURBT联合吡柔比星灌注治疗，对照组采用TURBT进行治疗；记录两组患者手术时间、置留导尿管时间，随访期间内膀胱穿孔、复发情况，随访时检测血清中VEGF-C、VEGF-B、VEGF-A、DKK-4、DKK-3、DKK-2、DKK-1、IL-2、PCNA、CA125蛋白水平和STAT5、STAT3、mTOR、JAK、AKT、PI3K mRNA水平。结果：观察组导尿管置留时间和临床复发率均显著低于对照组（P<0.05）；观察组患者VEGF-C、VEGF-B、VEGF-A、DKK-4、DKK-3、DKK-2、DKK-1、IL-2、PCNA、CA125水平均显著低于对照组（P<0.05）；观察组患者血清中STAT5、STAT3、mTOR、JAK、AKT、PI3K mRNA水平显著低于对照组；观察组患者不良反应发生率显著低于对照组，且差异存统计学意义（P<0.05）。结论：采用TURBT联合吡柔比星灌注治疗后，可有效降低浅表性膀胱癌患者的肿瘤细胞活力，下调mTOR信号通路因子表达，具有较好的临床疗效。

Purposes: To analyze the effect of TURBT combined with perfusion on tumor cell viability and the expression of mTOR signal pathway factor.Methods: One hundred thirteensuperficial bladder cancer cases admitted in our department between January 2010 and January 2015 were enrolled into observation group treated with TURBT combined with THP, and control group treated with TURBT; the operation time, indwelling catheterization time,the follow-up period, perforation of the bladder, recurrence, test serum VEGF-C, VEGF-B, VEGF-A, DKK-4, Dkk-3, DKK-2, DKK-1, IL-2, PCNA, CA125 protein levels and STAT5, STAT3, mTOR, JAK, Akt and PI3K mRNA levels were recorded. Results: In the observation group,catheter indwelling time and clinical recurrence rate were significantly lower than those in the control group (P<0.05); VEGF-C, VEGF-B, VEGF-A, DKK-4, Dkk-3, DKK-2, DKK-1, IL-2, PCNA, CA125 levels were significantly lower than that in the control group (P<0.05); STAT5, stat3, mTOR, JAK, Akt and PI3K mRNA levels were also significantly lower than those in control group; The incidence of adverse reactions in the observation group was significantly lower than that in the control group (P<0.05). Conclusions: TURBt combined with Pirarubicin perfusion in superficial bladder cancer patients can effectively reduce the tumor cell viability, down regulated mTOR signaling pathway factor expression.

参考文献：

[1] 平秦榕,颜汝平,王剑松,等.膀胱癌早期诊断方法的临床价值[J].医学与哲学,2015,36(6):66-68,97.
[2] DANHUA Z,GUAN Z,ZGUGA G,et al.c(RGDfK) decorated micellar drug delivery system for intravesical instilled chemotherapy of superficial bladder cancer[J].J Control Release,2013,169(3):204-210.
[3] 廖勇,黄建林,安宇,等.不同手术方式联合早期膀胱灌注化疗对浅表性膀胱癌患者生活质量的影响[J].四川医学,2013,34(6):763-765.
[4] 裴建强,杜宏纲.经尿道膀胱肿瘤电切术联合吡柔比星膀胱灌注治疗浅表性膀胱癌临床观察[J].山东医药,2013,53(39):41-42.
[5] BENEDICT W F,FISHER M,ZHANG X Q,et al.Use of monitoring levels of soluble forms of cytokeratin 18 in the urine of patients with superficial bladder cancer following intravesical Ad-IFNα/Syn3 treatment in a phase l study[J].Cancer Gene Ther,2014,21(3):91-94.
[6] 李涛,杨立,明星,等.浅表性膀胱癌经尿道术后早期灌注化疗临床预后的系统评估[J].循证医学,2013,13(3):147-151.
[7] RAJEEVE V,PEARCE W,CASCANTE M,et al.Polyamine production is downstream and upstream of oncogenic PI3K signalling and contributes to tumour cell growth[J].Biochem J,2013,450(Pt.3):619-628.
[8] 马晋,范地兵,廖凯,等.不同药物在膀胱灌注治疗浅表性膀胱癌中的效果比较[J].四川医学,2013,34(1):104-105.
[9] EBI H,COSTA C,FABER A C,et al.PI3K regulates MEK/ERK signaling in breast cancer via the Rac-GEF,P-Rex1[J].PNAS,2013,110(52):21124-21129.
[10] 伦雪萍,静进.浅表性膀胱癌患者应对方式与生存质量相关性研究[J].中华肿瘤防治杂志,2013,20(22):1763-1766.
[11] HARE L M,PHESSE T J,WARING P M et al.Physiological expression of the PI3K-activating mutation Pik3ca(H1047R) combines with Apc loss to promote development of invasive intestinal adenocarcinomas in mice[J].Biochem J,2014,458(Pt.2):251-258.
[12] WEI H C.A numerical study of a mathematical model of pulsed immunotherapy for superficial bladder cancer[J].Jpn J Ind Appl Math,2013,30(2):441-452.
[13] BAUSEK N,ZEIDLER M P.Gα73B is a downstream effector of JAK/STAT signalling and a regulator of rho1 in drosophila haematopoiesis[J].J Cell Sci,2014,127(1):101-110.
[14] ZHANG Y,YOU J,REN W,et al.Drosophila glypicans dally and dally-like are essential regulators for JAK/STAT signaling and unpaired distribution in eye development[J].Dev Biol,2013,375(1):23-32.
[15] ZHAO L,SHA Y Y,ZHAO Q,et al.Enhanced 5-fluorouracil cytotoxicity in high COX-2 expressing hepatocellular carcinoma cells by wogonin via the PI3K/Akt pathway[J].Biochem Cell Biol,2013,91(4):221-229.
[16] WANG H,TIBBITT M W,LANGER S J,et al.Hydrogels preserve native phenotypes of valvular fibroblasts through an elasticity-regulated PI3K/AKT pathway[J].PANS,2013,110(48):19336-19341.

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