Objective To analyze the individual program and related factors of prostate biopsy guided by ultrasound. Methods A retrospective analysis of our hospital 234 cases clinically suspected prostate cancer patients of first puncture biopsy, were divided into three groups, a group of 135 cases (based on the volume of prostate) is divided into: A1 group 73 cases (≤50 ml) by classical 6+x the puncture point scheme, A2 group 62 cases( >50ml) by 12+x puncture point square case; group B 70 cases (a randomized control group, regardless of size) are used 12+x puncture point scheme; C group 29 cases (>50ml) using classical 6+x puncture point scheme. Correlation analysis of puncture results. Results In 234 cases, 110 cases of prostate cancer were detected in cases (47%). Comparison of A1 and A2 group the positive biopsy rate, the difference is statistically significant (P < 0.05);A, B group of positive biopsy rate comparison of results, the difference was statistical significance (P < 0.05); A2, B group of positive biopsy rate comparison of results,and the differences are not statistically significant (P > 0.05); C group (29 cases),the serum levels of column prostate - specific antigen (PSA) >50ng/mL 17 cases, all detected for PCA; serum PSA is less than or equal to 50ng/mL12 cases detection of PCa in 2 cases. Conclusions According to the volume of prostate, serum PSA concentration and imaging findings, it is feasible to make individual program. Prostate volume is less than or equal to 50 ml of the patient should adopt a classic 6+x the puncture point scheme; volume >50ml patients. When the serum PSA>50ng/mL, combined with image characteristics of performance, the classic 6+x puncture point scheme when the serum PSA is less than or equal to 50ng/mL to the occasion with 12+x the puncture point scheme.