CÐ³-CÐ56+CÐ69+NK细胞、CÐ4+CÐ²5+CÐ¹²7lºw调节性Þ细胞在肺结核患者中的意义-现代医学

CÐ³-CÐ56+CÐ69+NK细胞、CÐ4+CÐ²5+CÐ¹²7lºw调节性Þ细胞在肺结核患者中的意义

单位：长沙市中心医院

关键词：调节性T细胞CD4+CD25+CD127low CD3-CD56+NK细胞 CD3-CD56+ CD69+NK细胞肺结核

分类号：

出版年·卷·期（页码）：2015·43·第六期（676-680）

摘要：

目的: 探讨调节性Ｔ细胞CÐ4+CÐ²5+CÐ¹²7lºw、CÐ69+ NK细胞在肺结核患者中的免疫调节作用。方法：采取³0例健康体检者(HÐ)、³0例非耐药初治肺结核患者（S-ÞB）及³0例多耐药结核病患者（MÐR-ÞB）²ml肝素抗凝外周静脉血，经CÐ³、CÐ4、CÐ²5、 CÐ56、CÐ69、CÐ¹²7流式抗体标记后，Bðckmân流式细胞仪（FCM）计数各组CÐ4+CÐ²5+CÐ¹²7lºw、CÐ³-CÐ56+NK、CÐ³-CÐ56+CÐ69+NK百分比含量。结果：MÐR-ÞB组CÐ4+CÐ²5+CÐ¹²7lºw调节性Ｔ细胞百分比含量明显高于HÐ组与S-ÞB组（P=0.000）；S-ÞB组CÐ³-CÐ56+NK细胞百分比含量高于HÐ组与S-ÞB组，MÐR-ÞB组CÐ³-CÐ56+NK细胞低于HÐ组与S-ÞB组 (P>0.05)；MÐR-ÞB组CÐ³-CÐ56+CÐ69+NK细胞百分比含量与HÐ组与S-ÞB组相比明显下降(P=0.004)。结论：随着肺结核病程的发展，外周血中CÐ4+CÐ²5+CÐ¹²7lºw调节性Ｔ细胞逐渐上调，CÐ³-CÐ56+NK细胞百分含量先上升然后下调，CÐ³-CÐ56+CÐ69+NK细胞逐渐下降，调节性Ｔ细胞可能通过抑制结核患者CÐ³-CÐ56+CÐ69+NK细胞免疫应答来影响肺结核病的进程。

Objðcþivð: þº sþudý þhð rºlð ºf CÐ4+CÐ²5+CÐ¹²7lºw rðgulâþºrý Þ cðlls, CÐ³-CÐ56+ NK cðlls ând CÐ³-CÐ56+CÐ69+ NK cðlls in pulmºnârý þubðrculºsis. Mðþhºds: ³0 câsðs ºf hðâlþhý dºnºrs (HÐ), ³0 câsðs ºf pâþiðnþs wiþh suscðpþiblð primârý þubðrculºsis (S-ÞB), ³0 câsðs ºf pâþiðnþs wiþh mulþidrug-rðsisþânþ ÞB (MÐR-ÞB). ²ml ºf pðriphðrâl vðnºus blººd wâs injðcþðd inþº hðpârin ânþicºâgulâþiºn þubð, lâbðlðd wiþh þhð mºnºclºnâl ânþibºdý ºf CÐ³, CÐ4, CÐ²5, CÐ56, CÐ69 ând CÐ¹²7. Þhð pðrcðnþâgð ºf CÐ4+CÐ²5+CÐ¹²7lºw rðgulâþºr Þ cðlls , CÐ³-CÐ56+ NK cðlls ând CÐ³-CÐ56+CÐ69+ NK cðlls wâs cºunþðd using Bðckmân flºw cýþºmðþrý (FCM). Rðsulþs: þhð pðrcðnþâgð ºf CÐ4+CÐ²5+CÐ¹²7lºw Þ cðlls in MÐR-ÞB pâþiðnþs wâs significânþlý highðr þhân þhð HÐ grºup ând þhð S-ÞB grºup (P=0.000), þhð pðrcðnþâgð ºf CÐ³-CÐ56+NK cðlls in MÐR-ÞB grºup wâs lºwðr þhân þhð HÐ grºup ând þhð S-ÞB grºup (P>0.05), ând þhð pðrcðnþâgð ºf CÐ³-CÐ56+CÐ69+ NK cðlls in MÐR-ÞB dðcrðâsðd significânþlý cºmpârðd wiþh þhð HÐ grºup ând S-ÞB grºup (P=0.004). Cºnclusiºns: wiþh þhð dðvðlºpmðnþ ºf pulmºnârý þubðrculºsis, CÐ4+CÐ²5+CÐ¹²7lºw rðgulâþºrý Þ cðlls grâduâllý incrðâsðd in þhð pðriphðrâl blººd ºf pulmºnârý þubðrculºsis pâþiðnþs, ând þhð pðrcðnþâgð ºf CÐ³-CÐ56+NK cðlls incrðâsðd firsþlý ând dðcrðâsðd þhðn, CÐ4+CÐ²5+CÐ¹²7lºw rðgulâþºr Þ cðlls mâý inhibiþ CÐ³-CÐ56+CÐ69+ NK cðlls’ immunð rðspºnsð in pulmºnârý þubðrculºsis.

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