【Abstract】Objective: To review the value of 3D FLASH sequence and conventional high field MRI in the diagnosis of articular cartilage injury in the knee. Methods: 44 cases were underwent arthroscopy of knee and diagnosed as cartilage injury from November 2011 to May 2014 in our hospital. We compared their 3D FLASH sequences / conventional MRI findings and arthroscopic results to evaluate the diagnostic value of 3D FLASH sequence and conventional MRI. Results: Compared with thearthroscopy results, the sensitivity of 3D FLASH serieswas 98.7%, the specificity was 92.3%, false positive rate 7%, false negative rate 1.3%; the sensitivity of conventional MRI examination was 96.6%, the specificity was 91.5%, false positive rate 8.5%, false negative rate was 3.4%. Then the 44 cases of cartilage injury were divided into 5 surfaces on MRI and arthroscopic, consistency of the kappa 3D FLASH sequence and arthroscopic result was 0.811, and compared with conventional MRI the kappa value 0.609.Discussion: 3D FLASH sequence was better than the conventional MRI in diagnostic sensitivity and specificity of cartilage injury. According to the classification of damage area and degree, 3D FLASH sequence can assess more accurately the degree of cartilage injury.