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PFGE对医院分离鲍曼不动杆菌的同源性分析
作者:闻海丰  
单位:河北医科大学第三医院
关键词:鲍曼不动杆菌 脉冲场凝胶电泳 同源性分析 
分类号:
出版年·卷·期(页码):2015·43·第四期(403-409)
摘要:

目的:了解鲍曼不动杆菌的临床分布特征、耐药特性,检测医院内不同病房鲍曼不动杆菌是否存在同源性,分析鲍曼不动杆菌的耐药情况和医院交叉感染的可能途径,以便更好的控制鲍曼不动杆菌在医院中传播。方法:收集河北医科大学第三医院2012年2月至2012年12月临床分离的55株鲍曼不动杆菌, 采用脉冲场凝胶电泳(Pulsed field gel electrophoresis,PFGE)方法对菌株进行同源性分析;采用K-B琼脂纸片扩散法测定鲍曼不动杆菌对临床常用抗菌药物的敏感性。结果:55株鲍曼不动杆菌经PFGE分型分为4个主要基因型,分别用A、B、C、D表示,其中A型33株, B型14株,C型4株,D型4株, A型和B型为主要的流行株,分布在多个不同的科室。ICU病房分离的菌株以A型和B型为主;骨科系统病区以B型为主;呼吸科病区以A型和C型为主。结论:医院内鲍曼不动杆菌存在多个基因型,初步判断存在院内交叉感染发生。

Objective: In order to investigate the clinical distribution and antibiotic susceptibility of clinical isolated Acinetobacter baumannii in hospital, all isolates were examined for the homology. We also analysis of antibiotic resistance and the possible transmission routes of hospital infection for control it spread in the hospital. Methods:55 isolates of Acinetobacter baumannii were collected from The Third Hospital of HeBei Medical University from February 2012 to December 2012. Analysis of the homology of all isolates by Pulsed field gel electrophoresis(PFGE) method. The susceptibility to different antibiotics was assessed with Kirby-Bauer disk diffusion method. Results: 55 isolates of Acinetobacter baumannii are typing by PFGE. Genotypic analysis of these isolates revealed 4 distinct patterns, A (n=33), B (n=14), C (n=4), D (n=4). Pattern A and B were the dominating clone, distributed in different wards. The isolates from ICU ward are mainly A type and B type. The isolates from department of orthopedics ward are mainly B type.The isolates from Department of respiration are mainly A type and C type. Conclusion: The isolates of Acinetobacter baumannii in hospital have multiple genotypes. There may exist Multidrug resistant Acinetobacter baumannii spread in hospital.

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