髓系抑制细胞及IL-23/Th17轴与儿童咳嗽变异性哮喘的相关性研究-现代医学

髓系抑制细胞及IL-23/Th17轴与儿童咳嗽变异性哮喘的相关性研究

单位：四川省眉山市人民医院

分类号：

出版年·卷·期（页码）：2015·43·第三期（336-339）

摘要：

摘要：目的：检测儿童咳嗽变异性哮喘（CVA）患者外周血髓系抑制细胞（MDSCs）、IL-23及IL-17的水平变化，初步探讨MDSCs及IL-23/Th17轴对CVA的影响。方法：随机选取自2011年10月至2013年10月85例被确诊为CVA的儿童患者为观察组，并根据哮喘严重程度分为CVA1~4级组，同时选取30例同期来本院行健康体检的儿童为对照组。应用流式细胞术检测各组MDSCs占外周血单个核细胞（PBMCs）的比例，ELISA法检测外周血清IL-23及IL-17水平。结果：与对照组相比，CVA组的MDSCs%、IL-23及IL-17均明显升高（P<0.05），其中CVA2~4级的IL-23和IL-17水平、CVA3~4级的MDSCs%显著升高（P<0.01）。CVA4级的MDSCs%、IL-23及IL-17水平均明显高于CVA1~2级（P<0.05），CVA3级的MDSCs%、IL-23及IL-17水平均明显高于CVA1级（P<0.05）；相关性分析可知，CVA 组MDSCs%与IL-23及IL-17均呈正相关（r=0.9655,0.9452; P=0.0012,0.001）。结论：随着CVA的严重程度逐渐加重，MDSCs、IL-23及IL-17水平明显升高，这表明MDSCs在CVA中可能起了重要的作用，其机制可能是MDSCs通过上调IL-23的表达水平，激活IL-23/Th-17轴，增加IL-17及其他炎症因子的表达分泌，从而加速了CVA慢性炎症的发展

Abstract: Objective: To study the effect of MDSCs, IL-23/Th17 axis on CVA through detected the levels of MDSCs, IL-23 and IL-17 in children with cough variant asthma (CVA), Methods: 85 patient children with CVA were randomly selected during 2011 OCT~2013 OCT as the observation group. The observation group was divided into four groups (CVA1~CVA4 grade group) according to the severity of asthma. The percentages of MDSCs on peripheral blood mononuclear cells (PBMCs) were detected by flow cytometry. The levels of IL-23 and IL-17 in serum were detected with enzyme inked immunosorbent assay (ELISA). Results: Compared with the control group, in the CVA groups MDSCs%, IL-23 and IL-17 were gradually increased（P<0.05）. The levels of IL-23 and IL-17 in CVA2~4 grade groups were dramatic increased（P<0.01）, and MDSCs% in CVA3~4 grade groups were also dramatic increased（P<0.01）. The levels of IL-23, IL-17 and MDSCs% in CVA 4 grade group were higher than those in CVA1~2 grade groups（P<0.05）. Also, The levels of IL-23, IL-17 and MDSCs% in CVA 3 grade group were higher than those in CVA1grade group（P<0.05）. Spearman rank correlation showed, MDSCs% and IL-23, IL-17 had the positive correlate in CVA groups(r=0.9655, 0.9452; P=0.0012, 0.001). Conclusion: The levels of IL-23, IL-17 and MDSCs were obviously increased with the severity of CVA gradually aggravated, that shows MDSCs may play an important role in the pathogenesis of CVA. The mechanism may be MDSCs up-regulating the accumulation of IL-23, activating the IL-23/ Th-17 axis, increasing the expression of IL-17 and other inflammatory factors, accelerating the development of CVA chronic inflammation.

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