Objective: To investigate clinical value of Color Doppler Ultrasound(CDUS) and Multi-slice Computed Tomography(MSCT) in comparative diagnosis of urothelial carcinoma. Methods: A retrospective analysis of the CDUS and MSCT data of 42 patients suffered bladder urothelial carcinoma was generated. Meanwhile, a comparative analysis between the numbers of tumor lesions in different parts of bladder calculated from two methods and detected from postoperative pathologic examination was performed.The CDUS and MSCT performance of the urothelial carcinoma of the bladder were concluded.Result: 92 sites were detected in 42 cases of patients with bladder cancer (59 sites in neck and triangle areas, 33 sites in other parts). Preoperative tumor detection rate MSCT (71.19%) of vesical neck and triangular area was higher than CDUS (44.07%), and the difference was statistically significant (p<0.05); the tumor detection rate MSCT (75.76%) of other parts was higher than CDUS (69.70%), but the difference was non- significant (p> 0.05). Do not distinguish the locations of lesions, MSCT detection rate (71.19%) was higher than the detection rate of CDUS (44.07%), and the difference was significant (p<0.05). The CDUS of the urothelial carcinoma of the bladder was mainly performed as nodositas, papillary or cauliflower-like protruding into the bladder lumen (69.39%) from the bladder wall, 35 tumor cases (71.43%) presented the moderate and uniform echo, and inside the tumor can be detected flow signal (83.67%). The MSCT of the urothelial carcinoma of the bladder was mainly performed as papillary or cauliflower -like mass (68.65%), and tumor bodies were mostly soft tissues with a density higher than urine (88.06%), the majority were above moderately strengthened post-enhancement (79.11%), and 57 cases (85.07%) with most tumors homogeneously enhanced.Conclusion: The urothelial carcinoma of the bladder has a more typical performance of CDUS and MSCT. CDUS and MSCT are both important means of dete