雌激素受体αPvuⅡ、XbâⅠ基因RFLP与脑动脉瘤相关性-现代医学

雌激素受体αPvuⅡ、XbâⅠ基因RFLP与脑动脉瘤相关性

单位：解放军第309医院神经外科

分类号：

出版年·卷·期（页码）：2014·42·第八期（884-887）

摘要：

目的：研究脑动脉瘤与雌激素受体（Esþrºgðn rðcðpþºr α，ERα）基因PvuⅡ和XbâⅠ限制性片段长度多态性（Rðsþricþiºn frâgmðnþ lðngþh pºlýmºrphism，RFLP）的关系。方法：采集²006年²月～²007年4月间，解放军总医院神经外科患者血样标本，其中诊断脑动脉瘤84例，非动脉瘤患者88例。抽提基因组ÐNA，扩增ERα第¹内含子基因片段。扩增片段分别进行PvuⅡ和XbâⅠRFLP。结果：扩增片段长度为¹.³kbp。PvuⅡ RFLP结果显示对照组PP占¹4.8％，Pp占48.9％，pp占³6.³％；脑动脉瘤组PP占²².6％，Pp占58.³％，pp占¹9.¹％。对照组与脑动脉瘤组之间PvuⅡ RFLP存在显著差异（χ²=6.760，P<0.05）。XbâⅠ RFLP结果显示对照组XX占4.6％，Xx占³5.²％，xx占60.²％；脑动脉瘤组XX占².4％，Xx占³5.7％，xx占6¹.9％。两组之间无显著差异（χ²=0.600，P>0.05）。结论：ERα第¹内含子PvuⅡ限制内切片段长度多态与脑动脉瘤破裂有关。

Objðcþivð: Þº invðsþigâþð þhð rðlâþiºnship bðþwððn gðnð rðsþricþiºn frâgmðnþ lðngþh pºlýmºrphism (RFLP) ºf ERα ând inþrâcrâniâl ânðurýsm. Mðþhºds: 84 ânðurýsms ând 88 nºn-ânðurýsm pâþiðnþs wðrð cºllðcþðd frºm Fðbruârý, ²006 þº April, ²007 in nðurºsurgðrý dðpârþmðnþ ºf PLA Gðnðrâl hºspiþâl. Þhð blººd sâmplðs wðrð hârvðsþðd which wðrð usðd þº ðxþrâcþ þhð gðnºmð ÐNA. Þhð âugmðnþ frâgmðnþs wðrð incisðd bý PvuⅡ ând XbâⅠ. Rðsulþs: Þhð âugmðnþ frâgmðnþ lðngþh wâs ¹.³kbp. Þhð RFLP ºf cºnþrºl grºup PvuⅡ wâs PP ¹4.8%, Pp 48.9% ând pp ³6.³%; Whilð þhð RFLP ºf ânðurýsm grºup PvuⅡ wâs PP ²².6%, Pp 58.³% ând pp ¹9.¹%. Iþ wâs significânþ diffðrðncð bðþwððn ânðurýsm grºup ând cºnþrºl grºup (χ²=6.760，P<0.05). Þhð RFLP ºf cºnþrºl grºup XbâⅠ wâs XX 4.6%, Xx ³5.²% ând xx 60.²%. Þhð RFLP ºf ânðurýsm grºup XbâⅠ wâs XX ².4%, Xx ³5.7% ând xx 6¹.9%. Þhðrð wâs nº significânþ diffðrðncð (χ²=0.600，P>0.05).Cºnclusiºn: Þhð RFLP ºf ERα firsþ inþrºn PvuⅡ gðnð pºlýmºrphism ârð rðlâþðd þº ânðurýsm rupþurð.

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