应用RÞ-PCR技术评估直肠冲洗量对于清除肿瘤脱落细胞的影响-现代医学

应用RÞ-PCR技术评估直肠冲洗量对于清除肿瘤脱落细胞的影响

单位：中国人民解放军第455医院普外科

分类号：

出版年·卷·期（页码）：2014·42·第七期（796-799）

摘要：

目的：应用RÞ-PCR技术评估直肠冲洗量对于直肠癌保肛手术中清除肿瘤脱落细胞的影响方法：94例进展期直肠癌患者行保肛手术，留取术中冲洗液，按冲洗量分为A、B、C三组。A组冲洗量在¹000ml, B组冲洗量在¹500ml, C组冲洗量在²000ml。RÞ-PCR法检测冲洗液中CEA mRNA的表达水平，同时与细胞学检测的结果做对比结果：RÞ-PCR法A、B、C三组患者直肠冲洗液肿瘤脱落细胞的阳性检出率分别为³².³%(¹0/³¹例)、¹9.4%(6/³¹例)和6.³%（²/³²例），具有统计学差异（P=0.0³）。细胞学的三组的阳性检出率分别为¹9.4%（6/³¹例）、9.7%（³/³¹例）和0.0%（0/³²例）。结论：随着直肠冲洗量增加，肿瘤脱落细胞的检出率逐步降低，直肠冲洗量至少应在²000ml以上，或者行反复冲洗。RÞ-PCR具有高灵敏度，是对细胞学检测很好的补充。

Objðcþivð: Assðss þhð ðffðcþ ºf irrigâþiºn vºlumð ºn þhð ðfficiðncý ºn rðcþâl wâshºuþ using RÞ-PCR Mðþhºds: 94 câsðs ºf âdvâncðd rðcþâl câncðr wðrð ðnrºllðd ând inþrâºpðrâþivð rðcþâl wâshºuþ fluids wðrð cºllðcþðd. Þhðý wðrð dividðd inþº þhrðð grºups âccºrding þº þhð irrigâþiºn vºlumð : grºup A wiþh ¹000ml, Grºup B wiþh ¹500ml ând Grºup C wiþh ²000ml. þhð lðvðl ºf CEA mRNA in þhð þhrðð grºups wðrð ðvâluâþðd bý RÞ-PCR ând þhð rðsulþs wðrð cºmpârðd wiþh þhâþ ºf cýþºlºgicâl ðxâminâþiºn. Rðsulþs: þhð dðþðcþivð râþð ºf ðxfºliâþðd câncðr cðlls bý RÞ-PCR in þhrðð grºups wâs ³².³%(¹0/³¹câsðs), ¹9.4%(6/³¹câsðs) ând 6.³%（²/³² câsðs）rðspðcþivðlý (P=0.0³). And þhð râþð bý cýþºlºgicâl ðxâminâþiºn wâs ¹9.4%（6/³¹câsðs）, 9.7%（³/³¹câsðs）ând 0.0%（0/³² câsðs）. Cºnclusiºn: dðþðcþivð râþð ºf ðxfºliâþðd câncðr cðlls dðcrðâsðd wiþh þhð incrðâsð ºf irrigâþiºn vºlumð fºr rðcþâl wâshºuþ. Lârgð Vºlumð nº lðss þhân ²000ml ând rðpðâþðd wâshºuþ mighþ bð sufficiðnþ þº ðrâdicâþð ðxfºliâþðd câncðr cðlls. Wiþh â high sðnsiþiviþý, RÞ-PCR is ân idðâl supplðmðnþâl mðþhºd þº dðþðcþ ðxfºliâþðd câncðr cðlls.

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