Ojective: We investigated the expression level of Tie-2 within 50 cases of endometrial carcinomas and corresponding normal paired endometrial tissues to evaluate the correlation between Tie-2 expression level and malignant biological behaviors of endometrial cancers, such as invasive and metastatic potential. Methods: Semi-quantitative reverse transcription polymerase chain reaction analysis was performed to assess Tie-2 mRNA expressions in 50 cases of endometrial carcinomas, as well as corresponding normal endometrial tissues, Tie-2 protein levels were also determined by Western blotting and immunohistochemistry was applied to locate intercellular position of Tie-2. Results: Increased levels of Tie-2 mRNA and protein were found in endometrial carcinoma tissues compared with corresponding normal endometrial tissues. Expression of Tie-2 gene was closely related to depth of invasion and lymph node metastasis. Significantly increased levels of Tie-2 in poorly differentiated and undifferentiated endometrial carcinoma were found, compared with well-differentiated and moderately differentiated adenocarcinoma. The immunoreactivity of Tie-2 was observed in the cytoplasm of tumor cell and blood vascular endothelium. Conclusions: Tie-2 mainly express in endometrial carcinoma cell and blood vascular endothelium and its expression of vary positively with differentiated stage of endometrial cancer cells. Overexpression of Tie-2 may involve invasion and metastasis progress of endometrial cancer.