Objective To study the pathogenic bacteria distribution of ascites and drug sensitivity analysis in liver cirrhosis with spontaneous bacterial peritonitis. Methods Retrospective analysis the clinical data of the 78 cases of liver cirrhosis patients with spontaneous bacterial peritonitis from June 2011 to June 2013,including gender, age, their corresponding bacterial distribution and drug sensitivity. Result 31 pathogens were isolated from 78 cases and the isolation rate was 39.74%,ascites infection with G-bacterial distribution, accounting for detection of bacteria in 61.3%,isolation rate of the previous five pathogens were Escherichiacoli, Staphylococcus aureus, Klebsiella, Coagulase-negative staphylococcus. Conclusion Liver Cirrhosis Patients susceptible to spontaneous bacterial peritonitis, a reasonable choice should be based on susceptibility of antibiotics, and should take effective preventive measures to reduce spontaneous bacterial peritonitis.