Objective: investigate the effective dose of laparoscopy combined with methotrexate, providing reference for the clinical treatment of ectopic pregnancy. Methods:165 cases of patients with ectopic pregnancy in our hospital between June，2008 and June，2012 were selected and divided into low dose group there were 56 cases, treating with laparoscopic conservative operation combined with low dose MTX ; middle dose group there were 59 cases , treating with laparoscopic conservative operation combined with middle dose MTX; high dose group there were 50 cases, treating with laparoscopic conservative operation combined with high dose MTX.the serum β -HCG level at before treatment , third day,seventh day, fourteenth day after treatment and the recovery time of serum β –HCG, intrauterine pregnancy rate and PEP rate in follow-uped two years.Results: the serum β –HCG at postoperative third day,seventh day, fourteenth day in middle dose group were significantly lower than that in low dose group, the recovery time of serum β –HCG was significantly shortened;the serum β –HCG at postoperative third day,seventh day, fourteenth day in high dose group was significantly lower than that in low dose group and that inmiddle dose group,the recovery time of serum β –HCG was shorter than the other two groups.the PEP rate in three groups during two follow-uped two years were not statistically significant (P>0.05);the intrauterine pregnancy rate in high dose group or middle dose group was significantly higher than that of low dose group (P<0.05). Adverse reaction incidence among three groups had statistically significant difference (P<0.05), and that in high dose group was significantly higher than that in middle dose group or that in low dose group, but there was no obvious difference between in middle dose group and in low dose group (P>0.05). Conclusion: Treating ectopic pregnancy with laparoscopic operation combined with middle doses of MTX can effectively improve clinical