Objective: To investigate the amelioration of sorafenib on liver fibrosis in rat models. Methods: Liver fibrosis was induced in Wistar rats by intraperitoneal injection of DMN. Rats were gavaged with sorafenib in high dose (10mg/kg) and low dose (3mg/kg). Histological changes were observed by H&E staining, Masson’s trichrome staining and Sirius Red staining. Expression of α-SMA were detected by Immunohistochemistry. Results: The surface of the liver tissue in control group and low dose group appeared solitary or multiple, fibrosis nodules with different sizes. liver fibrosis is significantly ameliorated in high dose group compared to the control group and low dose group. Cell degeneration, necrosis, fibrosis, intrahepatic extensive pseudolobules fibrosis were visible in control group and low dose group by light microscope. No significant degeneration of the liver cells and regenerative nodules in high-dose group were detected. Collagen staining area of collagen staining area between control group and low dose group had no significant difference. Collagen staining area of the high-dose group was 52% or 49% of the control group or low dose group respectively, with significant differences compared to the staining area in control group. α-SMA expression in the control group and low dose group increased significantly compare to expression in high dose group.