Objective: to evaluate cystic arteries using 64-slice computed tomography angiography in the preoperative laparoscopic cholecystectomy. Methods: 87 patients were undergone abdominal computed tomographic angiography, all data were post-processed, and cystic arteries were reconstructed. It was assessed that the ability of displayed clearly cystic arteries with 64-slice computed tomography angiography, the region and branches of cystic artery, and the relation of cystic artery with Calot's Triangle were observed. Results: Cystic arteries in 84 patients were clearly shown the origin, branch and track of cystic arteries, accounting for 96.55% in all cases. 76 patients had the normal origin of cystic artery, accounting for 90.48%; 8 had origin variation. 78 cases had single branch of cystic artery, and 6 had two branches or more of cystic arteries; the cystic arteries in 74 patients located in Calot's Triangle, 8 the outside of Calot's Triangle, and 2 the inside and outside in Calot's Triangle. Conclusion: 64-slice computed tomography angiography is able to display fully the origin, branch and track of cystic artery, and further providing reliable imaging evidence for assessment of cystic artery in the preoperative laparoscopic cholecystectomy.