Abstract Objective Design and construction for the gene therapy of pancreatic carcinoma is the key target genes ( human NF-κB ) miRNA interference vector. Methods According to the design and synthesis of target genes ( miRNA oligo primer design software ), oligo annealing twin chain, then the vector construction kit for recombinant clones, double chain miRNA oligo inserted into expression vector construction of miRNA, miRNA plasmid, transformed into competent cells from DH5 conversion plate respectively, were cloned, use carrier universal primers of colony PCR screening, screening positive clones were sequenced, to verify the recombinational cloning insertion sequence and oligo sequence alignment design. Results After alignment, recombinant clone insert fragment sequences and oligo sequences identical design. Conclusion We have successfully constructed aiming at target genes ( human NF-κB ) miRNA interference carrier, laid the foundation for further study. |