Objective：To evaluate the effects of mesenchymal stem cells on the migration of pancreatic cancer in mice. Methods：The mesenchymal stem cells (MSCs) from green fluorescent protein(GFP) transgenes were separated and purified by Ficoll density gradient centrifugation and adherent in vitro being identified by detecting their surface markers with flow cytometry. The migration of MSCs labeled with GFP to pancreatic cancer cells Panc-1 was determined with Transwell chamber in vitro. The pancreatic cancer model in nude mice was established, and GFP-MSCs were injected into the caudal veins. Then the tumor-bearing mice were sacrificed at 1d, 4d, 7d, 10d, 14d, 17d, and 20d after treatment, and the tumors, livers, pancreas, lung, kidney and splen were retrieved for rapid frozen section and investigation of GFP-MSCs in each organizations by fluorescence microscope. Results：The MSCs were obtained successfully by Ficoll density gradient centrifugation and adherent as more than 90% of total cells were CD44+CD45-、CD90+CD45- cells by flow cytometry. Migration of GFP-MSCs to pancreatic cancer cells Panc-1 was observed in Transwell chamber test, and this migration was enhanced with the increase of pancreatic cancer cells Panc-1(P＜0.05). For the tumor-bearing mice, GFP-MSCs were targeted and accumulated into the pancreatic tumors in vivo. In the first 10 days after administration, the level of GFP-MSCs in the tumors raised over time(P＜0.05), but there was no signigicantly increase after 10 d.(P＞0.05).Colclusion：MSCs would migrated towards pancreatic tumor cells in vitro, and they would directionally accumulated in pancreatic tumor in vivo.