[Abstract] Objective To estabilish liver fibrosis mode of different stage in rats and observe the trending of hemodynamic change during the development of liver fibrosis by CEUS. To study the value of CEUS in the noninjury diagnosis of liver fibrosis. Methods Sixty SD rats were divided to two groups randomly, 8 as control group, 52 for liver fibrosis mode.HAAT,PVAT,HVAT,and HA-HVTT(HVAT-HAAT) were recorded with CEUS.In CnTI program obtain the time-intensity curve of liver parenchyma and record the time to peak , peak intensity, ascending slope, descent slope.All rats were grouped according to pathological liver fibrosis stage. ROC analysis was performed to assess the value of these methords on diagnosis of liver fibrosis(S≥l) and early liver cirrhosis (S=4). Results ROC analysis showed AUC of HA-HVTT was 0.792 and ROC curve was not intersected with diagonal reference line, the diagnostic value was moderate. AUC of HVAT、HA-HVTT for diagnosing early liver cirrhosis (S=4) were all bigger than 0.9, the diagnostic accuracy was high; the AUC of peak intensity and descent slope for diagnosing early liver cirrhosis was 0.828 and 0.852, the diagnostic value was moderate.Conclusions CEUS can be used to observe the developing process of the hepatic artery，portal vein，hepatic vein. HA-HVTT was helpful for evaluating liver fibrosis(S≥1) and early liver cirrhosis(S=4) and it is promising that HA-HVTT will become a significant imaging index in quantitative diagnosis of liver cirrhosis.