To investigate the best timing to be treated with hemoperfusion for sepsis rabbits by detecting the dynamic concentrations of IL6 and TNFα. Methods: 18 rabbits were randomly divided into 6 groups. They were treated with hemoperfusion once respectively in 3 hours, 6 hours, 12 hours, 24 hours, 48 hours after the cecal ligature and puncture surgery, accompanied the establishment of air perfusion group (control group). The blood concentration of IL6 and TNF-α were dynamically respectively detected before the surgery, after 3 hours, 6 hours, 9 hours, 12 hours, 24 hours, 48 hours. Then we drawn the curves and noted down the rabbit survival time. Results: The average concentration of blood IL6 in 12 hours’ hemoperfusion group was the lowest, accompanying the longest survival time (P<0.05); Nevertheless, The average concentration of blood TNF-αin 3 hours’ hemoperfusion group was the lowest（P<0.05）, accompanying the declining of the peak, but not prolonging the survival time. Conclusion: Hemoperfusion in the early-term(3 hours) and medium-term progress of sepsis (24 hours) can separately effectively reduce the blood concentration of inflammatory mediator TNF-αand IL6, and the later one can make the experiment rabbits’ survival time longest, instructing the decision of our clinical hemoperfusion timing.