不同剂量致敏原对小鼠哮喘模型中气道反应性的变化-现代医学

不同剂量致敏原对小鼠哮喘模型中气道反应性的变化

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出版年·卷·期（页码）：2011·39·第二期（121-125）

摘要：

【摘要】目的探讨不同剂量致敏原鸡卵蛋白（OVA）制备哮喘模型时对哮喘小鼠气道反应性是否有影响。方法 30只BALB/c小鼠随机分为哮喘组和正常对照组（C组）,哮喘组又分为小剂量OVA致敏组（A组）和大剂量OVA致敏组(B组)，每组各10只。A、B组在0、14d时给予含卵蛋白(OVA)的致敏液200ul（A组含10ug OVA,B组含2mg OVA，两者含同样的氢氧化铝及生理盐水）,21d开始雾化吸入OVA，连续雾化 6d,操作时观察小鼠活动及呼吸情况；各组分别于末次雾化激发12h后测定小鼠的无创肺功能中的增强呼气间歇(Penh)值以评价气道反应性；对支气管肺泡灌洗液(BALF)行细胞总数、嗜酸性粒细胞计数；取肺组织作HE染色病理切片；ELISA方法测外周血、BALF上清液中的IgE、IFN-gama含量；结果 ①哮喘B组在第二次致敏后可见喘息，呼吸困难，口唇和尾巴粘膜发紫表现，并雾化时出现扭体，燥动，呼吸加快等表现。②哮喘A组、B组的Penh值明显高于正常对照组,p<0.01;从Mch 6.25mg/ml开始时哮喘B组的Penh值明显高于哮喘A组，P<0.01; ③哮喘A组、B组的BALF中细胞总数（2.41±0.90; 4.89±3.49）及嗜酸性料细胞数（0.93±0.18;1.74±0.76）较正常对照组明显增高（0.65±0.34 ,P<0.05;0.30±0.16,P<0.05）；哮喘B组BALF中细胞总数、嗜酸性粒细胞均较哮喘A组多，但P>0.05。④哮喘A、B组的支气管、血管周围，肺间质及肺泡腔内可见嗜酸性粒细胞、淋巴细胞浸润，气道上皮损伤，结构紊乱，气道内杯状细胞肥大增生，气道内分泌大量黏液并有粘液栓形成。正常对照组肺组织气道结构清晰，气道纤毛上皮排列整齐，无明显的炎症改变。⑤哮喘A、B组外周血及BALF中的IgE含量较正常组增多，两者P<0.01；哮喘B组外周血和BALF中IgE（37.47±6.18，26.10±7.18）较哮喘A组（26.09±3.76，P<0.01；12.47±3.02，P<0.01）明显增多。⑥哮喘A、B组外周血及BALF中的IFN-gama含量较正常组减少，两者P<0.01；哮喘B组外周血和BALF中IFN-gama含量（35.29±10.92，37.44±21.01）较哮喘A组（32.22±10.04，P>0.05；36.89±22.00，P>0.05）增多。结论：哮喘小鼠组模型制备成功；较高剂量OVA致敏哮喘模型较低剂量OVA致敏的气道高反应性更敏感，在观察气道高反应方面，OVA=2mg组致敏的哮喘模型优于常用致敏剂量OVA=10ug组。

【Abstract】Objective To explore the different does of Ovalbumin(OVA) sensitization whether play a fluence in airway hyperresponsiveness of mice model of asthma. Methods　The mice were randomly divided into 3 groups ,with 10 mice in each group: control group and asthmatic groups(including asthma A group ,the does OVA sensitization is 2 mg every time ; and asthma B group,which is 10 ug ). The asthma A、B group were sensitized in the 0th、14th day with different does of OVA each other ,then inhaled ovalbumin(OVA)beginning the 21st day ,and lasting for 6 days . to observe the breathing and the color of labiali and tail mucosa.The last time OVA stimulateed, after 12 hours ,the mice were measured the Penh to evaluate the airway responsiveness by Noninvasive lung functional instrument , bronchoalveolar lavage cytology was performed and lung tissue sections were prepared for histopathologic examination to evaluate the airway inflammation．examine the IgE、IFN-gama of blood and BALF by ELISA method. Results①the skin of mice lip and tail was caynosis after the second allergic sensitization in asthmatic B group mice.②The airway hyperrespomsiveness in asthmatic group was significantly higher than that in the control mice,and asthmatic B group`s Penh was significantly higher than asthmatic A group; ③The total cell number and the eosinophils in BALF of the asthmatic mice were significantly higher than that in the control mice ( both P< 0.05)；The total cell number and the eosinophils in BALF of the asthmatic B group were higher than that in asthmatic A group , but p>0.05; ④the contents of IgE in peripheral blood and BALF of the asthmatic mice were significantly higer than that in the control mice, P<0.01,and he asthmatic B group （37.47±6.18，26.10±7.18）were higher than that in asthmatic A group （26.09±3.76，P<0.01；12.47±3.02，P<0.01）.⑤ the contents of IFN-gama in peripheral blood and BALF of the asthmatic mice were lower than that in control ones, and the asthmati

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