Objective:To investigate the effect of Ginsenoside Rg1(GRg1) on the expression of mucin 5AC(MUC5AC) induced by lipopolysaccharide(LPS) in human nasal epithelial cells HNEpC, and to elucidate its possible mechanism. Methods: Human nasal mucosal epithelial cells HNEpC were treated with different concentrations of LPS or GRg1. The proliferation activity of cells was detected by CCK-8, and the intervention concentrations of LPS and GRg1 were confirmed. HNEpC cells were treated with 400 ng·ml-1 LPS or 40 μmol·L-1 GRg1 respectively or simultaneously, and the cells were divided into blank control(Blank) group, LPS group, LPS+GRg1 group, LPS+3-MA group and LPS+GRg1+3-MA group. The content of MUC5AC in the supernatant was detected by ELISA; the expression level of MUC5AC mRNA in cells was detected by qRT-PCR; the apoptosis rate was measured by Annexin V/PI staining method; the aggregation of autophagic vesicles was detected by Monodansyl cadaverine(MDC) staining; the formation of autophagosomes was observed under transmission electron microscope, and the expression levels of MUC5AC, Beclin 1, LC3 Ⅰ and LC3Ⅱ proteins were detected by Western blot. Results: Compared with the Blank group, the expression levels of MUC5AC mRNA and protein in the cells of the LPS group, and the MUC5AC content in the supernatant were significantly increased(P<0.01), the apoptosis rate was increased(P<0.01), the autophagy level increased, and the expression levels of autophagy-related proteins Beclin 1 and the LC3Ⅱ/Ⅰ ratio were markedly increased(P<0.01). Compared with the LPS group, the expression levels of MUC5AC mRNA and protein expression in the cells of the LPS+GRg1 group and LPS+3-MA group, and the content of MUC5AC in the supernatant were significantly reduced(P<0.01), the apoptosis rate was decreased(P<0.01), the autophagy level in the cells was decreased, and the expression level of autophagy-related proteins Beclin 1 and LC3Ⅱ/Ⅰ ratio were markedly decreased(P<0.01). Compared with the LPS+3-MA group, the LPS+3-MA+GRg1 group had no significant changes in the above indicators of cells(P>0.05). Conclusion: Ginsenoside Rg1 can inhibit the high expression of MUC5AC in human nasal epithelial cells induced by LPS, and at the same time inhibit cell apoptosis. The mechanism may be related to the inhibition of cell autophagy. |
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