Objective: To investigate radiosensitizing effect of glycyrrhetinic acid on SW579 cells and its possible mechanism. Methods: MTT assay was used to detect the inhibition rate of cell growth. Apoptosis was detected by flow cytometry and protein expression of LC3-Ⅰ and LC3Ⅱ was detected by Western bloting. Results: After treated with 5, 10, 20, 40, and 80 μmol·L-1 GA, the growth inhibition rates of SW579 cells were (4.64±0.83)%, (18.37±3.11)%, (33.54±6.32)%, (48.24±7.16)% and (82.32±12.23)%, respectively, with significant differences between the groups(P<0.001). The growth inhibition rate of 5 μmol·L-1 GA combined with 2, 4, 8, 16 and 32 Gy radiation treatment was significantly higher than that of 2, 4, 8, 16 and 32 Gy radiation treatment alone(P<0.01). The apoptosis rate of SW579 cells in 5 μmol·L-1 GA+8 Gy radiation treatment group was significantly higher than that in 8 Gy radiation treatment group (P<0.01). The ratio of LC3Ⅱ/LC3-Ⅰ in 8 Gy radiation treatment group was significantly higher than that in control group (P<0.01). The ratio of LC3Ⅱ/LC3-Ⅰ in 5 μmol·L-1 GA+8 Gy radiation treatment group was significantly lower than that in 8 Gy radiation treatment group (P<0.01). Conclusion: Glycyrrhetinic acid can inhibit autophagy induced by radiation and increase the sensitivity of thyroid cancer cells to radiation. |
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