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甘草次酸对甲状腺癌SW579细胞放射敏感性的影响
作者:田甜  莫淑婵  吴钟彪  江聪 
单位:海南省海口市中医医院 外科, 海南 海口 570216
关键词:甘草次酸 甲状腺癌 放疗敏感性 自噬 
分类号:R965.2
出版年·卷·期(页码):2020·48·第十一期(1381-1384)
摘要:

目的:研究甘草次酸(glycyrrhetinic acid,GA)对甲状腺癌SW579细胞的放射增敏作用,以及其作用的可能机制。方法:四氮噻唑蓝(MTT)细胞毒实验检测细胞生长抑制率,流式细胞术检测细胞凋亡,蛋白质免疫印迹法(Western bloting)检测自噬蛋白LC3-Ⅰ和LC3Ⅱ表达。结果:MTT细胞毒实验结果显示,5、10、20、40、80 μmol·L-1 GA处理甲状腺癌SW579细胞,生长抑制率分别为(4.64±0.83)%、(18.37±3.11)%、(33.54±6.32)%、(48.24±7.16)%和(82.32±12.23)%,各组间差异具有统计学意义(P<0.01)。5 μmol·L-1 GA联合2、4、8、16、32 Gy射线处理细胞的抑制率显著高于单独的2、4、8、16、32 Gy射线处理细胞的抑制率(P<0.01)。流式细胞术凋亡检测结果显示,5 μmol·L-1 GA+8 Gy射线组的SW579细胞凋亡率显著高于8 Gy射线组(P<0.001)。Western bloting结果显示,8 Gy射线组LC3Ⅱ/LC3-Ⅰ值显著高于对照组(P<0.01),5 μmol·L-1 GA+8 Gy射线组LC3Ⅱ/LC3-Ⅰ值显著低于8 Gy射线组(P<0.01)。结论:GA可以抑制放射线激发的甲状腺癌细胞自噬增强,显著增加甲状腺癌细胞对放射线的敏感性。

Objective: To investigate radiosensitizing effect of glycyrrhetinic acid on SW579 cells and its possible mechanism. Methods: MTT assay was used to detect the inhibition rate of cell growth. Apoptosis was detected by flow cytometry and protein expression of LC3-Ⅰ and LC3Ⅱ was detected by Western bloting. Results: After treated with 5, 10, 20, 40, and 80 μmol·L-1 GA, the growth inhibition rates of SW579 cells were (4.64±0.83)%, (18.37±3.11)%, (33.54±6.32)%, (48.24±7.16)% and (82.32±12.23)%, respectively, with significant differences between the groups(P<0.001). The growth inhibition rate of 5 μmol·L-1 GA combined with 2, 4, 8, 16 and 32 Gy radiation treatment was significantly higher than that of 2, 4, 8, 16 and 32 Gy radiation treatment alone(P<0.01). The apoptosis rate of SW579 cells in 5 μmol·L-1 GA+8 Gy radiation treatment group was significantly higher than that in 8 Gy radiation treatment group (P<0.01). The ratio of LC3Ⅱ/LC3-Ⅰ in 8 Gy radiation treatment group was significantly higher than that in control group (P<0.01). The ratio of LC3Ⅱ/LC3-Ⅰ in 5 μmol·L-1 GA+8 Gy radiation treatment group was significantly lower than that in 8 Gy radiation treatment group (P<0.01). Conclusion: Glycyrrhetinic acid can inhibit autophagy induced by radiation and increase the sensitivity of thyroid cancer cells to radiation.

参考文献:

[1] 殷德涛,刘晨光.甲状腺癌的放射治疗[J].中国实用外科杂志,2019,39(3):206-208.
[2] 陈焕杰,许洁,李存琪,等.甲状腺癌外放射治疗研究进展[J].中华普通外科学文献(电子版),2019,13(1):72-75.
[3] 黄清波.冬凌草甲素对甲状腺癌细胞放射敏感性的影响及机制[J].中国老年学杂志,2020,40(1):185-190.
[4] LI J J,TANG F,LI R K,et al.Dietary compound glycyrrhetinic acid suppresses tumor angiogenesis and growth by modulating antiangiogenic and proapoptotic pathways in vitro and in vivo[J].J Nutr Biochem,2020,77(1):1-13.
[5] 智信,陈晓,苏佳灿.甘草次酸药理作用研究进展[J].现代中西医结合杂志,2019,28(25):2847-2850.
[6] 赵永强,田德增,魏晓华,等.姜黄素增强人乳头瘤状甲状腺癌细胞TP C-1放射敏感性的研究[J].中华放射肿瘤学杂志,2016,25(8):886-890.
[7] 张光辉,龙旭,张拴,等.甘草次酸的结构修饰及生物活性研究进展[J].当代化工,2020,49(2):393-397.
[8] 王若宁,柳雨影,陈健,等.甘草酸、甘草次酸的抗肿瘤机制及其作为药物递送载体的研究进展[J].中草药,2019,50(23):5876-5886.
[9] 李凤岩,陈亚光,林大勇,等.甘草次酸对甲状腺癌细胞SW579凋亡的影响及其机制[J].中国应用生理学杂志,2019,35(5):464-467.
[10] LI M,WANG Y,JIANG S H,et al.Biodistribution and biocompatibility of glycyrrhetinic acid and galactose-modified chitosan nanoparticles as a novel targeting vehicle for hepatocellular carcinoma[J].Nanomedicine (London,England),2020,15(2):145-161.
[11] 宋冠初,刘丹,郭根燕,等.自噬对放射敏感性的影响[J].中华放射肿瘤学杂志,2019,28(12):953-956.
[12] 邓军容,黄伟,易红,等.谷氨酰胺分解代谢促进鼻咽癌自噬和放疗抵抗性[J].中国医师杂志,2018,20(6):820-825.
[13] 张美婷,山顺林,耿炜.癌细胞放射抗拒性机制的研究进展[J].东南大学学报(医学版),2017,36(1):116-119.
[14] 刘艾芸,乔久涛,陈晶,等.NPRL2对结肠癌HT29细胞系自噬和凋亡的影响[J].东南大学学报(医学版),2019,38(2):249-255.
[15] DAVID D,ANNETT L,ANNA D.SLC3A2/CD98hc,autophagy and tumor radioresistance:a link confirmed[J].Autophagy,2019,15(10):1850-1851.

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