Objective:To investigate the diagnostic value of urinary kidney injury moleculer-1 (uKIM-1) and urinary liver fatty acid binding protein (uL-FABP) in spesis patients with acute kidney injury (AKI). Methods:A total of 165 spesis patients from February 2016 to February 2018 were involved, and they were divided into AKI group (n=81) and non-AKI group (n=84)according to whether AKI occured within 24 hours after ICU accepted. The uKIM-1 and uL-FABP levels were tested with enzyme-linked immunosorbent assay(ELISA). The correlations between uL-FABP (or uKIM-1) and clinical parameters were analyzed, and diagnostic values of uKIM-1 and uL-FABP for AKI were calculated by receiver operating characteristic curve(ROC).Results: The uKIM-1[(6.16±2.71)vs. (3.37±1.29)ng·mg-1, P<0.001]and uL-FABP[(465.11±193.54)vs. (255.51±87.99) ng·mg-1, P<0.001] levels in AKI group were both higher than those in non-AKI group. In different AKI stage, uKIM-1 and uL-FABP levels in patients of AKI-3 stage[(8.06±2.62)ng·mg-1, (602.42±196.74)ng·mg-1]were higher than those of AKI-2[(6.30±2.26)ng·mg-1, (449.27±148.77)ng·mg-1] and AKI-1[(4.67±2.22)ng·mg-1, (379.29±171.89)ng·mg-1] stage. In AKI group, the uKIM-1 level was positively correlated with sCr (r=0.42, P<0.05), lactic acid(r=0.55, P<0.05), SOFA score(rs=0.25, P<0.05) and AKI stage(rs=0.46, P<0.05), and negatively correlated with eGFR(r=-0.31, P<0.05); the uL-FABP level was positively correlated with sCr (r=0.34, P<0.05), lactic acid(r=0.68, P<0.05), SOFA score(r=0.30, P<0.05) and AKI stage (rs=0.51, P<0.05), and negatively correlated with eGFR(r=-0.23, P<0.05). Area under curve(AUC) of uKIM-1 and uL-FABP used for the diagnosis of AKI in spesispatients was 0.83 and 0.81, and AUC of uKIM-1 combined with uL-FABP was 0.88. AUC of uKIM-1 and uL-FABP used to distinguish AKI-3 from AKI-1/2 was 0.77 and 0.78, and AUC of uKIM-1 combined with uL-FABP was 0.82.Conclusion: The uKIM-1 and uL-FABP levels are evaluatedin sepsis patients with AKI, and the two parameters can be used for AKI stage diagnosis.
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