Objective: To explore the clinical value of different biomarkers in the diagnosis of osteosarcoma.Methods: 56 patients with osteosarcoma from May 2014 to December 2017 diagnosed in our hospital (osteosarcoma group), 62 patients with orthopedic benign hyperplasia (benign disease group) and 62 cases of healthy subjects (control group) within the same period of time and of age were selected. T cell immunoglobulin and mucin domain-3(Tim-3), microRNA-21(miR-21), alkaline phosphatase (ALP), lactate dehydrogenase (LDH)level in the three groups were comparatively analyzed, receiver operating curve (ROC) was drawn and the diagnostic efficacy of Tim-3, miR-21, ALP, LDH alone and in combination with the diagnosis of osteosarcoma were evaluated. Results: There was a statistically significant difference in levels of Tim-3, miR-21, ALP, LDH in the three groups (P<0.05). The ROC curve showed that the AUC of combined Tim-3 and miR-21 was 0.929, it was significantly higher than that of single diagnosis. The optimal cut-off point of Tim-3 was 12.37 ng·ml-1, miR-21 was 1.60 copies·μl-1, ALP was 239.84 U·L-1, LDH was 266.91 U·L-1. Using the above cut-off points, the sensitivity and positive predictive value of combined diagnosis were significantly higher than single diagnosis(P<0.05).Conclusion: Tim-3 combined with miR-21 has a higher value in the diagnosis of osteosarcoma, wherein Tim-3 ≥ 12.37 ng·ml-1, miR-21 ≥ 1.60 copies·μl-1 can be regarded as the best cut-off point for osteosarcoma screening. |
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