人绒毛膜促性腺激素对乳腺癌大鼠肿瘤生长及相关基因表达的影响-现代医学

人绒毛膜促性腺激素对乳腺癌大鼠肿瘤生长及相关基因表达的影响

单位：1. 株洲市中心医院病理科, 湖南株洲 412000;
2. 株洲市中心医院急诊科, 湖南株洲 412000;
3. 南华大学肿瘤研究所, 湖南衡阳 421001

分类号：R-33;R737.9

出版年·卷·期（页码）：2020·39·第三期（303-307）

摘要：

目的：探讨人绒毛膜促性腺激素（human chorionic gonadotropin，hCG）对乳腺癌大鼠肿瘤生长及相关基因表达的影响。方法：将未经生产的40只雌性SD大鼠随机分为对照组和hCG干预组，每组20只。观察两组大鼠乳腺肿瘤的发生与生长情况，采用荧光定量PCR技术检测大鼠乳腺癌细胞以及其他正常乳腺组织中与乳腺癌相关基因的表达情况。结果：对照组大鼠的致癌例数、肿瘤数量显著高于hCG干预组（P<0.05），潜伏期短于hCG干预组（P<0.05）。hCG干预组大鼠MMP1、MMP2、MMP9、VEGF、IGF1基因表达低于对照组（P<0.05），TIMP1、PTEN、IGEIR基因表达高于对照组（P<0.05）。结论：hCG对乳腺癌大鼠的肿瘤生长起到抑制作用，对TIMP1、PTEN、IGF1R基因的表达起促进作用，对MMP1、MMP2、MMP9、VEGF、IGF1基因的表达起到抑制作用。

Objective: To investigate the effect of human chorionic gonadotropin (hCG) on tumor growth and related gene expression in breast cancer rats. Methods: Unproduced 40 female SD rats were randomly divided into control group and human chorionic gonadotropin intervention group,20 in each. The occurrence and growth of breast tumors in the two groups of rats were observed. Fluorescence quantitative PCR was used to observe the expression of genes associated with breast cancer in rat breast cancer cells and other normal breast tissues. Results: The number of carcinogens and the number of tumors in the control group were significantly higher than that in the hCG intervention group (P<0.05), and the latency time was longer than that of the hCG intervention group (P<0.05). The expressions of MMP1, MMP2, MMP9 and vascular endothelial growth factor(VEGF)in the hCG intervention group were lower than those of the control group (P<0.05), and the expression of the TIMP1 gene was higher than that of the control group (P<0.05). The expression of PTEN and IGF1R genes in the hCG intervention group was significantly higher than that in the control group, and the expression of IGF1 gene was lower than that in the control group (P<0.05). Conclusion: hCG inhibits the growth of breast cancer in rats and promotes the expression of TIMP1, PTEN and IGF1R, and inhibits the expression of RNA as well as IGF1, such as MMP1, MMP2, MMP9, VEGF and so on.

参考文献：

[1] 李雪英,周静,罗军,等.血清β-hCG水平动态变化与滋养细胞侵入输卵管壁深度的相关性研究[J].东南大学学报(医学版),2015,34(5):754-757.
[2] SZCZERBA A,LIWA A,KUBICZAK M,et al.Human chorionic gonadotropin β subunit affects the expression of apoptosis-regulating factors in ovarian cancer[J].Oncol Rep,2016,35(1):538-539.
[3] 徐少元,李洁,郭鲁燕,等.HCG对人子宫内膜细胞VEGF表达调控的初步研究[J].现代妇产科进展,2015,24(12):903-906.
[4] 彭书敏."βhCG-ERK1/2-MMP-2"信号通路调控卵巢癌细胞侵袭转移的作用及机制研究[D].石河子:石河子大学,2017.
[5] 南永刚,许建林,李楠,等.CA153、CA125联合HER-2检测在乳腺癌各临床分期诊断中的应用[J].现代肿瘤医学,2016,24(19):3070-3073.
[6] 魏兰,苏敏,黄云秀,等.PKM2基因沉默对人乳腺癌MCF-7细胞增殖及侵袭的影响[J].肿瘤,2016,35(9):968-975.
[7] RAO C V.Protective effects of human chorionic gonadotropin against breast cancer:how can we use this information to prevent/treat the disease?[J].Reprod Sci,2017,24(8):1102-1103.
[8] MIN S,XU X,WEI W,et al.Involvement of human chorionic gonadotropin in regulating vasculogenic mimicry and hypoxia-inducible factor-1α expression in ovarian cancer cells[J].Cancer Cell Int,2016,16(1):50-51.
[9] 万丽丽,刘洋,刘丽桃,等.人乳腺癌中VWCE基因的表达及其功能研究[J].肿瘤,2017,37(7):742-749.
[10] LI J,YIN M,SONG W,et al.B subunit of human chorionic gonadotropin promotes tumor invasion and predicts poor prognosis of early-stage colorectal cancer[J].Cell Physiol Biochem,2018,45(1):237-238.
[11] RAFIEE M,PANAHI Y,ALIKHANI M Y,et al.Concentration of alpha fetoprotein and beta-human chorionic gonadotropin tumor markers in sulfur mustard-exposed veterans[J].Int J Occup Environ Med,2017,8(3):184-185.
[12] 华亚男,牟文凤,宋金莲,等.沉默RRS1基因表达对乳腺癌BT549细胞生物学功能的影响[J].肿瘤,2018,38(1):35-43.
[13] 王丽,覃庆洪,谭启杏,等.干扰FBI-1表达对人乳腺癌细胞增殖的影响及其作用机制研究[J].中国癌症杂志,2017,27(4):262-267.
[14] 余天雾,龚建平,郭丹,等.乳腺癌肿大腋窝淋巴结中BCSCs相关基因表达与肿瘤复发、转移的关系[J].重庆医学,2016,45(9):1223-1227.
[15] TAPIA-PIZARRO A,ARGANDOñA F,PALOMINO W A,et al.Human chorionic gonadotropin (hCG) modulation of TIMP1 secretion by human endometrial stromal cells facilitates extravillous trophoblast invasion in vitro[J].Hum Reprod,2013,28(8):2215-2227.
[16] FORSTNER D,MANINGER S,NONN O,et al.Platelet-derived factors impair placental chorionic gonadotropin beta-subunit synthesis[J/OL].J Mol Med (Berl),2019-12-20.doi:10.1007/s00109-019-01866-x.
[17] ZHANG Z,HUANG Y,ZHANG J,et al.Activation of NF-κB signaling pathway during HCG-induced VEGF expression in luteal cells[J].Cell Biol Int,2019,43(3):344-349.

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录