S100A8和S100A9对鼻咽癌CNE-2细胞基质金属蛋白酶的影响-现代医学

S100A8和S100A9对鼻咽癌CNE-2细胞基质金属蛋白酶的影响

单位：1. 鄂东医疗集团市中心医院肿瘤内科, 湖北黄石 435000;
2. 武汉大学人民医院耳鼻咽喉科, 湖北武汉 430060

分类号：R739.63

出版年·卷·期（页码）：2019·47·第十二期（1446-1451）

摘要：

目的： 探讨S100A8和S100A9对鼻咽癌CNE-2细胞基质金属蛋白酶（MMP）的影响。方法： 构建S100A8及S100A9的siRNA载体、重组pEGFP-N1-S100A8及重组pEGFP-N1-S100A9过表达载体，将siRNA载体及过表达载体转染至鼻咽癌CNE-2细胞，分别与siRNA对照组、过表达对照组及空白对照组进行比较。观察各组细胞转染效率，检测细胞中MMP7、MMP9、MMP12的基因及蛋白表达水平。结果： 细胞转染效率为60%~70%。S100A8-siRNA组、S100A9-siRNA组细胞中MMP7、MMP9、MMP12的表达水平均较siRNA对照组、过表达对照组及空白对照组明显下降，差异均具有统计学意义（P<0.05）。S100A8过表达组、S100A9过表达组细胞中MMP7、MMP9、MMP12的表达水平均较各对照组明显上升，差异均具有统计学意义（P<0.05）。结论： 高表达的S100A8和S100A9可能促进MMP7、MMP9、MMP12的表达，与鼻咽癌的侵袭、转移有一定的关系。

Objective: To investigate the effect of S100A8 and S100A9 on matrix metalloproteinase (MMP) in nasopharyngeal carcinoma CNE-2 cells. Methods: Recombinant S100A8 and S100A9 siRNA vectors,pEGFP-N1-S100A8 and pEGFP-N1-S100A9 overexpression vectors were constructed and transfected into nasopharyngeal carcinoma CNE-2 cells.The transfection efficiency of each group was observed.The gene and protein expression levels of MMP7, MMP9 and MMP12 in each group were detected.The results of S100A8 siRNA group, S100A9 siRNA group,S100A8 overexpression group,S100A9 overexpression group were compared with the siRNA control group, overexpression control group and normal control group. Results: The transfection efficiency was 60%-70%. The expression levels of MMP7, MMP9 and MMP12 in S100A8-siRNA group and S100A9-siRNA group were significantly lower than those in the siRNA control group, overexpression control group and blank control group(P<0.05).The expression levels of MMP7, MMP9 and MMP12 in S100A8 overexpression group and S100A9 overexpression group were significantly higher than those in the control groups(P<0.05). Conclusion: The high expression of S100A8 and S100A9 may promote the expression of MMP7, MMP9 and MMP12, which may be related to the invasion and metastasis of nasopharyngeal carcinoma.

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