富硒酵母和小球藻复合物对ICR小鼠免疫力的影响-现代医学

富硒酵母和小球藻复合物对ICR小鼠免疫力的影响

作者：郑立新¹ 蔡祥焜¹ 乔秋爽¹ 刘颖芬² 辛乃宏² 李炳乾² 吴玉平¹ 田世民¹

单位：1. 中国检验检疫科学研究院综合检测中心食品药品安全评价中心, 原国家质检总局毒理重点实验室, 北京 100176;
2. 中盐工程技术研究院有限公司, 天津 300450

分类号：R392-33

出版年·卷·期（页码）：2019·47·第六期（636-640）

摘要：

目的：探讨富硒酵母和小球藻复合物对小鼠免疫力的影响。方法：健康ICR小鼠240只分成4组，每组60只，组内设3个剂量组和1个对照组，以0.375、0.750、2.250 g·kg^-1·d^-1连续灌胃30 d后，分别进行迟发型变态反应试验、半数溶血值测定和抗体生成细胞检测、碳廓清试验、吞噬鸡红细胞试验、NK细胞活性检测和ConA诱导小鼠脾淋巴细胞转化试验。结果：3个剂量组足趾肿胀度均明显高于对照组，差异均有统计学意义（P<0.01）；中剂量组淋巴细胞增殖能力明显高于对照组，差异有统计学意义（P<0.01）；低、高剂量组小鼠的抗体生成细胞数明显高于对照组，差异均有统计学意义（P<0.05）；高剂量组小鼠半数溶血值（HC₅₀）明显高于对照组，差异具有统计学意义（P<0.01）；高剂量组小鼠的吞噬率、吞噬指数明显高于对照组，差异具有统计学意义（P<0.01）；其他指标差异均无统计学意义（P>0.05）。结论：富硒酵母和小球藻复合物具有增强免疫力的功能。

Objective:To study the impact of a combination of selenium-enriched yeast and chlorella compound on the immunity function in ICR mice. Methods:A total of 240 ICR mice were randomly divided into 4 batches, each batch consisted of 3 dosage groups (0.375, 0.750 and 2.250 g·kg^-1·d^-1) and negative control group. After administered with the compound for 30 days by intragastric infusion, the mice were subjected to delayed type hypersensitivity (DTH), serum hemolysin determination(HC₅₀) and antibody producing cell detection, mice carbon clearance test, mice peritoneal macrophage phagocytosis test, NK cell activity assay and ConA-induced lymphocyte transformation test, respectively. Results:The results showed that swelling degree of toes of 3 dose groups were higher than that of negative control group, the capacity of the CONA-induced lymphocyte proliferation of middle dose group was stronger than that of the negative control group, the difference was statistically significant(P<0.01). The quantity of antibody forming cells of middle dose group and high dose group were higher than that of negative control group, the difference was statistically significant(P<0.05). The level of HC₅₀ of high dose group was higher than that of negative control group, the difference was statistically significant(P<0.01). The phagocytosis rate and phagocytosis index of macrophage cell in abdominal cavity of high dose group were higher than that of negative control group, the difference was statistically significant (P<0.01). However, the compound had no statistically difference on other indicators.Conclusion:The selenium-enriched yeast and chlorella compound can strengthen immunity function of mice.

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