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miR-145和CTGF在肺腺癌中的表达关系及意义
作者:段亚男  胡智慧  董英辉  党荣广  胡跃云  赵新  张燕 
单位:石家庄市第一医院 肿瘤一科, 河北 石家庄 050011
关键词:miR-145 结缔组织生长因子 肺腺癌 临床意义 
分类号:R734.2
出版年·卷·期(页码):2019·47·第三期(299-304)
摘要:

目的:探讨微小RNA-145(miR-145)、结缔组织生长因子(CTGF)在肺腺癌中的表达关系及其临床意义。方法:收集2012年6月至2014年3月于我院诊治并经病理证实的肺腺癌组织标本95例,同时取癌旁正常肺组织为正常组(95例)。实时定量聚合酶链反应(qRT-PCR)法检测肺腺组织中miR-145与CTGF表达量,免疫组化法检测肺腺组织中CTGF蛋白表达,根据检测结果将miR-145分为高表达组与低表达组,CTGF分为阳性表达组与阴性表达组,分析miR-145、CTGF表达与肺腺癌临床病理参数的关系,Pearson法对miR-145与CTGF进行相关性分析,Kaplan-Meier法对肺腺癌患者的生存情况进行分析。结果:肺腺癌组织中miR-145表达量显著低于正常组(P<0.05),而CTGF表达量显著高于正常组(P<0.05),且肺腺癌组织中CTGF蛋白阳性率显著高于正常组(P<0.05);miR-145、CTGF表达均与临床分期、淋巴结转移、TNM分期、病理分级、胸膜浸润显著相关(P<0.05);Pearson相关性分析结果显示miR-145与CTGF呈显著负相关(r=-0.512,P<0.05);Kaplan-Meier曲线显示肺腺组织中miR-145高表达组无病生存期(DFS)、总生存期(OS)显著高于低表达组,CTGF阴性表达组DFS、OS均显著高于阳性表达组,差异均具有统计学意义(P<0.05)。结论:与正常组相比,肺腺癌中miR-145下调表达而CTGF上调表达,两者呈负相关且与预后有关,可作为早期诊断肺腺癌以及评定患者预后的指标。

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