Objective:To evaluate the role of resveratrol on human renal proximal tubular cells HK-2 injury induced by high glucose and hypoxia/reoxygenation and the its possible relationship with TXNIP-NLRP3 pathway.Methods:HK-2 cell were randomly divided into 3 groups:the high glucose group (group HG),the high glucose and hypoxia/reoxygenation group(group HHR) and the high glucose and hypoxia/reoxygenation+resveratrol group(group HHR-RES),each n=5.The high glucose and hypoxia/reoxygenation model was established by stimulated with high glucose for 72 h,then exposed to hypoxia 4 h and reoxygenation 2 h. In the HHR-RES group, 50 μmol·L-1 of resveratrol was treated during the 72 h of high glucose incubation. The cell viability and cell damage was detected by CCK-8 and LDH respectively, oxidative stress was detected by SOD and MDA, the apoptotic rate of cells was detected by flow cytometry,the immunofluorescence staining was performed to detect the expression of TXNIP and NLRP3. Results:Compared with the group HG, the levels of LDH and MDA,apoptotic rate,the expression of TXNIP and NLRP3 were increased(all P<0.05);cell viability and SOD activity were decreased in the group HHR (all P<0.05),Compared with the group HHR,the levels of LDH and MDA, apoptotic rate,the expression of TXNIP and NLRP3 were decreased(all P<0.05);cell viability and SOD activity were increased in the group HHR+RES (all P<0.05). Conclusion:Resveratrol may inhibit TXNIP-NLRP3 pathway to alleviate the HK-2 cell injury induced by high glucose and hypoxia/reoxygenation.
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